Raman microscope with ultra-high speed imaging capability
- Only a few minutes of acquisition time is required for Raman imaging.
- Clear observation of fine structure by high spatial resolution of 0.3micro
at x, y direction and 0.7micro at z direction.
- Easy to learn and use software for all users.
- High peak position accuracy (0.1 cm-1) enabling to measure the strain of
- One simple mouse click enabling to measure any location on the sample
observed with CCD camera.
- Small footprint by compact design.
Outline of RAMAN-11
RAMAN-11 is a next generation laser microscope observing an
image of a sample with Raman scattering lights emitted from the molecules. Raman
spectroscopy technique used for RAMAN-11 gives researchers the knowledge for the
details of constituent elements of organelles or protein. By observing the Raman
scattering lights emitted from the molecules of the living cells, distribution
of the molecules can be imaged with the colors (wavelength) unique to those
molecules without using the staining method.
When a molecule is
illuminated with a monochromatic laser beam, a portion of the incident light is
scattered with different color. This scattered light is called "Raman
scattering" for the name of the discoverer. The Raman scattered light tells us
about the molecular is specific to the molecule, like a fingerprint. In addition,
the Raman scattered light from a crystal gives the lattice condition of the
crystal such as distortion, defects and impurity, and therefore can be utilized
for crystal analysis.
Due to the weakness of Raman scattered light,
conventional micro Raman system took a long time for one point measurement and
scanned the sample point by point to take images. The scanning speed of this
method is very slow. RAMAN-11 has noteworthy capable to accelerate the imaging
speed. Nanophoton's original technology of a flying spot scanning technique
contributes 100 times faster image acquisition than conventional
Examples of observation
|A Raman image of fullerene(C60) schowing the intensity peak at 1470cm-1. The fullerene distribution generated on the substrate is observed.
||Raman observation image of a paint surface. With an unknown mixture of substances, the surface state and the anamorphism can be observed and analyzed at the microscopic scale.
|Raman image of non-stained living cardiomyocytes. Raman peaks of lipid, protein, and nucleic acid are assigned to red, green and blue. Subcellular organelles and bio-molecules are observed.
||Phase contrast image(left) and Raman Image(center) of a white blood cell capturing foreign body.
Nonophoton's Laser Raman Microscope took a large step as RAMANplus. The RAMANplus has a built-in video rate confocal reflection system that acquires the three-dimensional surface profile very fast in advance of Raman measurenment. After the Raman measurement, the RAMANplus automatically appends the Raman chemical information to the three-dimensional profile.
- Quick measurement of three-dimensional surface profile
- Quick search of interesting points in bumpy samples
- Ultra-fast and high-definition Raman imaging
Easy link of chemical information to 3D-surface profile
- Video-rate confocal reflection microscopy
- Parallel detection system of Raman spectra
Laser beam scanning system
Observation example of Diamond file
The reflected light confocal observation gives the precise extended focus reflected image (grey color). The Raman observation gives the data of the crystal quality. These data are acquired and overlaid automatically.
Green: High quality diamond
Yellow: Low quality diamond